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Image Search Results
Journal: Blood Advances
Article Title: Hyperfiltration predicts long-term renal outcomes in humanized sickle cell mice
doi: 10.1182/bloodadvances.2018028878
Figure Lengend Snippet: Measures of glomerular injury in male and female HbSS and HbAA mice. Trajectory of urinary albumin excretion in male (A) and female (B) HbAA and HbSS mice. Trajectory of urinary nephrin excretion in male (C) and female (D) HbAA and HbSS mice. Quantification of glomerulosclerosis represented as sclerosis index score in male (E) and female (F) HbAA and HbSS mice. Quantification of glomerular vascular congestion represented as the sum of the glomerular congestion index score in male (G) and female (H) HbAA and HbSS mice. Quantification of the Bowman's capsule basement membrane thickening score in male (I) and female (J) HbAA and HbSS mice. Number of glomeruli per field in male (K) and female (L) HbAA and HbSS mice. Glomerular size represented as mean area of glomeruli from male (M) and female (N) HbAA and HbSS mice. (O) Representative Masson trichrome– and hematoxylin and eosin–stained sections of glomeruli from male HbAA and HbSS mice. Original magnification ×40; scale bar = 50 μm. (P) Representative Masson trichrome– and hematoxylin and eosin—stained sections of glomeruli from female HbAA and HbSS mice. Original magnification ×40; scale bars = 50 μm. Data are mean ± SEM; *P < .05 vs 8-week HbSS mice. Analysis by 1-way ANOVA with repeated measurements with Tukey post hoc analysis. (A) Genotype and age, P < .0001. (B) Genotype, P < .0001; age, P = .9348. (C) Genotype, P < .0001; age, P = .0202. (D) Genotype, P < .0001; age, P = .0279, or 2-way ANOVA with Tukey post hoc test. (E) Interaction, genotype, and age, P < .0001. (F) Interaction, P = .0039; genotype and age, P < .0001. (G) Interaction, P = .3446; genotype, P < .0001; age, P = .2662. (H) Interaction, P = .0003; genotype, P = .0002; age, P < .0001. (I-) Interaction, genotype, and age, P < .0001. (K) Interaction, P = .0571; genotype, P = .0050; age, P = .0038. (L) Interaction, P = .0032; genotype, P = .9450; age, P = .0009. (M) Interaction, P = .0118; genotype and age, P < .0001. (N) Interaction, P = .0005; genotype, P = .0006; age, P = .0001.
Article Snippet: Urinary nephrin concentration was measured using a
Techniques: Membrane, Staining
Journal: Blood Advances
Article Title: Hyperfiltration predicts long-term renal outcomes in humanized sickle cell mice
doi: 10.1182/bloodadvances.2018028878
Figure Lengend Snippet: Data showing the time course of glomerular injury in male and female HbSS and HbAA mice. (A) Quantification of WT-1+–stained glomerular sections from male HbAA and HbSS mice represented as number of WT-1+–stained cells per glomerulus. (B) Relative glomerular podocin expression in glomeruli isolated from male HbAA and HbSS mice. (C) Relative glomerular nephrin expression in glomeruli isolated from male HbAA And HbSS mice. (D) Quantification of WT-1+–stained glomerular sections from female HbAA and HbSS mice represented as number of WT-1+–stained cells per glomerulus. (E) Relative glomerular podocin expression in glomeruli isolated from female HbAA and HbSS mice. (F) Relative glomerular nephrin expression in glomeruli isolated from female HbAA and HbSS mice. (G) Representative WT-1+–stained sections of glomeruli from male and female HbAA and HbSS mice. Original magnification ×40; scale bars = 50 μm. Data are mean ± SEM; *P < .05 vs 8-week HbSS; #P < .05 vs 8-week HbAA. Analysis with 2-way ANOVA with Tukey post hoc analysis. (A) Interaction, genotype, and age, P < .0001. (B) Interaction and age, P < .0001; genotype, P = .4236. (C) Interaction, P = .0028; genotype, P < .0001; age, P = .0739. (D) Interaction and genotype, P < .0001; age, P = .0004. (E) Interaction, P = .2687; genotype, P = .0039; age, P = .0017. (F) Interaction, P = .5020; genotype, P = 9953; age, P = .0411.
Article Snippet: Urinary nephrin concentration was measured using a
Techniques: Staining, Expressing, Isolation